Improvement of serological diagnostics of poultry methanevmovirus infection
Abstract
An epizootological survey of metapneumovirus infection prevalence (MPVI) was conducted at poultry enterprises of Kharkiv district among “BIG-6” and “BUT-8” turkeys aged 4‒480 days (imported from Germany and Hungary), “Large White” turkeys aged 70 days, “Birkivska Barvysta” and “Hisex Brown” hens aged 250 and 180 days respectively. Using ELISA-based serology tests (“IDEXX” test-kit, USA), it was demonstrated that 78,0 % of the turkeys (antibody titers 3776‒27869), 100 % of the “Birkivska Barvysta” (4997‒10414) and 0 % of the “Hisex Brown” hens exhibited a positive reaction to MPVI. At Luhansk NAU the development of a domestic ELISA test-system for the diagnosis of MPVI in hens and turkeys is being conducted. To receive diagnostic sera it was developed an immunization scheme for intact chickens and turkeys also it was obtained two series of Hyperimmune serum with antibody ELISA titer 1:1700 in chickens and in turkeys 1:2800 (IDEXX). The sera were stored at the minus 20 °C temperature and in a freeze-dried state; technological characteristics of this system have been determined and compared to its imported analog. The 100 % correlation dependence is observed according to the diagnosticum data, it means that, the sera of the investigated birds, which do not have antibodies to this disease, are not fixed in both sets and vice versa, the serums with the presence of protective antibody titers to this disease - are detected by two systems used to test. The domestic test system is much cheaper, in comparison with the foreign analogues. It is available to veterinary medicine laboratories and is also competitive and import-substituting. The prospect of further research in this area is the introduction of a domestic ELISA test system to the MPVІ diagnosis in the veterinary medicine laboratory in Ukraine.References
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