Isolation and identification of epizootic culture Pasteurella multocida subspecio gallicida strain SA-18
Abstract
In the study of the etiopathogenesis of infectious pathology in 3-month-old vivarium chickens, proceeding according to the classical type without relay agent transmission, it was established that the etiophactor is P. multocida. The disease was characterized by septic pathogenesis, acute course with a lethal outcome, without pathognomonic postmorbid changes. One of the diseased chickens was killed with a diagnostic purpose in the agonized state and standard pathological material was selected. As a result of bacteriological study, we isolated an epizootic variant of the pasteurella identified by the complex of features as P. multocida sb. gallicida strain SA-18, which induced lethal pasteurellosis in vivarium chickens. The culture of the pathogen was pathogenic and highly virulent for laboratory animals, possessed cardinal biological properties characteristic for the species and subspecies, and at the same time gave a high yield of prokaryotes in stationary cultivation on commonly used nutrient media. Culture P. multocida sb. gallicida strain SA-18 was found to be pathogenic and highly virulent for laboratory animals ‒ white mice, antlers, rabbits and 3-month-old chicks, with a biological study according to generally accepted methods. For all laboratory animals, experimental pasteurellosis was an unconditional lethal infection, which proceeded sharply, the prognosis with respect to life was negative, leading sign of pathogenesis was sepsis. That is, infectious pathology proceeded according to the classical type of infectious process. However, in morlyans, sepsis was also accompanied by pneumoenteric disease with a lethal outcome, that is, pathogenesis had a transient nature and contained signs of classical and factor types of the infectious process of pasteurellosis. Isolated culture on a set of biological properties proved to be a promising model for the creation of inactivated bacterin in the polyvalent antipasteasterase vaccine, covering the entire antigenic spectrum of the field pathogen circulating in native conditions. The culture of the pathogen must be studied in relation to its suitability for the production of vaccine anti-pasteurized biologics. It is necessary to study the immunogenicity of P. multocida sb. gallicida strain SA-18 in the form of inactivated bacterium with and without adjuvant, kinetics of chemical inactivation of prokaryotes and the potential of accumulation of bacterial mass of pasteurells for suspension culture in a bioreactor.References
Апатенко В.М. Проблема мажорного патогена в экологии паразитоценозов на примере P. multocida / В.М. Апатенко, А.И. Сосницкий, В.П. Заболотная // Болезни диких животных: тр. междунар. науч.–практ. конф. (Покров, 28–30 сент. 2004 г.). – Покров, 2004. – С. 215–222.
Ашмарин И.П. Статистические методы в микробиологических исследованиях / И.П. Ашмарин, А.А. Воробьев. – Л.: Изд-во мед. лит-ры, 1962. – 182 с.
Джупина С.И. Рациональная эпизоотологическая классификация инфекционных болезней сельскохозяйственных животных / С.И. Джупина // Вестник РАСХН. – 2001. – № 2. – С. 71–75.
Джупина С.И. Факторные инфекции – современная проблема в эпизоотологии / С.И. Джупина // Аграрний вісник Причорномор’я. – Одеса: АТЗТ ІРЕНТ, 2009. – Вип. 21 – С. 24‒31.
Елинов Н.П. Общие закономерности строения и развития микробов–продуцентов биологически активных веществ / Н.П. Елинов. – Л.: Медицина, 1977. – 288 с.
Інфекційні та інвазійні хвороби кролів / [Л.Є Корнієнко, О.Б. Домбровський, С.І Пономар та ін.]. – Біла Церква, 2003. – 288 с.
Лабораторная диагностика инфекционных болезней животных / [А.А. Шевченко, Л.В. Шевченко, О.Ю. Черных и др.]. – Краснодар, 2009. – 575 с.
Лабораторні методи досліджень у біології, тваринництві та ветеринарній медицині: довідник / [В.В. Влізло, Р.С. Федорук, І.Б. Ратич та ін.]; за ред. В.В. Влізла. – Львів: СПОЛОМ, 2012. – 764 с.
Патент на корисну модель № 7439 UA, МПК 7А61К 39/00. Епізоотичний штам № 12 Pasteurella multocida серовар А для виготовлення вакцини проти факторного (ендогенного) пастерельозу телят і поросят, емульсійної інактивованої / Б.Т. Стегній, В.П. Заболотня, О.І. Сосницький; заявник та патентовласник Нац. наук. центр “Ін-т експерим. і клін. вет. медицини”. – № 2004120400; заявл. 17.02.04; опубл. 15.06.05, Бюл. № 6. – 4 с.
Паразитоценозы животных: учебное пособие / [А.Ф. Руденко, А.И. Сосницкий, А.А. Руденко и др.]. – Луганск: Элтон-2, 2014. – 590 с.
Стегний Б.Т. Методологические аспекты количественного определения Pasteurella multocida в суспензии / Б.Т. Стегний, А.И. Сосницкий // Вет. медицина: міжвід. темат. наук. зб. – Харків, 2008. – Вип. 91. – С. 454–457.
Brothers M.C. Membrane interaction of Pasteurella multocida toxin involves sphingomyelin / M.C. Brothers, M. Ho, R. Maharjan [et al.] // FEBS J. – 2011. – Vol. 278(23) – P. 4633–4648.
Haemorrhagic septicemia / OIE Manual of Diagnostic and Tests and Vaccines for Terrestial Animals Fifth Edition, 2004. – Vol. 1. – P. 537–548.
Miyoshi S. Pasteurella multocida pneumonia: zoonotic transmission confirmed by molecular epidemiological analysis / S. Miyoshi, H. Hamada, A. Miyoshi et al. // Geriatr. Gerontol. Int. – 2012. – № 12(1). – Р. 159–163.
Apatenko, V.M., Sosnytskyi, A.I., Zabolotnaya, V.P. (2004). The problem of the major pathogen in the ecology of parasitocenosis on the example of P. multocida. Diseases of wild animals: tr. Intern. scientific–practical. Conf. (Pokrov, September 28–30, 2004). Pokrov, 215–222.
Ashmarin, I.P., Vorob’ov, A.A. (1962). Statistical methods in microbiological studies. Leningrad, 182.
Jupina, S.I. (2001). Rational epizootological classification of infectious diseases of agricultural animals. Vestnik RAAS, 2, 71–75.
Jupina, S.I. (2009). Factor infections – a modern problem in epizootiology. Odesa: ATZT ІRENT, 21, 24–31.
Elinov, N.P. (1977). General patterns of the structure and development of microbes–producers of biologically active substances. Leningrad: Medicine, 288.
[Kornienko, L.E., Dombrovsky, O.B., Ponomar, S.I. et al.]. (2003). Infectious and invasive diseases of rabbits. Belaya Tserkov, 288.
[Shevchenko, A.A., Shevchenko, L.V., Chernykh, O.Yu. et al.]. (2009). Laboratory diagnostics of laboratory animal diseases. Krasnodar, 575.
[Vlizlo, V.V., Frowned, V.V., Fedoruk, R.S., Ratich, I.B. et al.]. (2012). Laboratory methods of research in biology, livestock and veterinary medicine: handbook. Lviv: SPOL, 764.
Stegny, B.T. Zabolotnya, V.P., Sosnytskyi O.I. (2005). Patent for Utility Model № 7439 UA, МПК 7А61К 39/00. Epizootic strain № 12 Pasteurella multocida serovar A for the production of a vaccine against factor (endogenous) pasterelosis of calves and piglets, emulsion inactivated. 6, 4.
[Rudenko, A.F., Sosnytskyi, A.I., Rudenko, A.A. et al.]. (2014). Parasitocenoses of animals: a manual for agrarian establishments. Lugansk: Elton–2, 590.
Stegny, B.T., Sosnytskyi, A.I. (2008). Methodological aspects of quantitative determination of Pasteurella multocida in suspension. Harkov, 91, 454–457.
Brothers, M.C., Maharjan, R. (2011). Membrane interaction of Pasteurella multocida toxin involves sphingomyelin. FEBS J. 278(23), 4633–4648.
Haemorrhagic septicemia (2004). OIE Manual of Diagnostic and Tests and Vaccines for Terrestial Animals Fifth Edition.1, 537–548.
Miyoshi, S., Hamada, H., Miyoshi, A. (2012). Pasteurella multocida pneumonia: zoonotic transmission confirmed by molecular epidemiological analysis. Geriatr Gerontol Int. 12, 1, 159–163.
