The content of fatty acids in egg yolks and quail embryo liver at different levels of tocopherol in feed

  • V. Trach
  • V. Danchuk
  • S. Midyk
  • V. Ushkalov
Keywords: quail, fatty acids, yolk, liver, vitamin E

Abstract

The article presents new scientific data on the characteristics of the fatty acid composition of the yolk fertilized quail eggs and liver tissues of the 14-day embryos of the quail of the breed of Pharaoh (Coturnix japonica). The influence of an additional setting on the vitamin E (in a dose of 20 g/tonn) on the fatty acid composition of liver tissues of 14-day quail embryos has been proved. Additional addition to the diet of the mother tocopherol population at a dose of 20 g/tonn contributed to an increase in the percentage of palmitic (1,44 %; p < 0,05) and stearic (0,52 %; p <0,05) acids in liver tissues embryos Reducing the total content of mono- and polyunsaturated fatty acids in the liver of embryos of 14-day quail for an additional introduction into the diet of the breeding stock of vitamin E is accompanied by a significant increase in the ratio of the amount of saturated to the amount of unsaturated fatty acids (NLC / NNHC) by 10.8% (p <0,05). The ratio of ω-6/ω-3 polyunsaturated fatty acids in yolk fertilized quail eggs is 15.3:1, whereas in the liver 14-day embryos 3.2–3.4: 1. There was a tendency to increase the share of ω-3 fatty acids (by 0,09 %) and decrease the share of ω-6 and ω-9 fatty acids in 0,97–1,07 % in the liver of quail embryos of the experimental group, resulting in the ratio of ω-6/ω-3 polyunsaturated fatty acids was 5,3 % less than control.

References

Бреславець В.О. Вплив розчинів гіпохлориту натрію та оцтової кислоти на ембріональний розвиток та виводимість яєць курей / В.О. Бреславець, Н.В. Шоміна, Ю.Р. Князєв // Птахівництво: міжвід. темат. наук. зб. – Харків, 2005. – Вип. 56. – С. 25‒35.

Scott T.A. Screening sanitizing agents and methods of application for hatching eggs I. Environmental and user friendliness / T.A. Scott, C. Swetnam // Journal of Applied Poultry Research. – 1993. – Т. 2, № 1. – P. 1‒6.

Khyzhnyak S.V. Fatty acids composition of inner mitochondrial membrane of rat cardiomyocytes and hepatocytes during hypoxia – hypercapnia / S.V. Khyzhnyak, S.V. Midyk, S.V. Sysoliatin // The Ukrainian Biochemical Journal. – 2016. – № 88(3). – P. 92–98.

Жирнокислотний склад сосисок торговельних мереж м. Київ / С.В. Мідик, В.О. Ушкалов, В.В. Данчук, С.В. Сисолятін, А.П. Нікітова // Ветеринарна біотехнологія. – 2018. – Т. 32, № 2. – С. 373–382.

Динаміка рухової активності свиней за впливу аквананохелатів та міцелярної форми токоферолу / В.В. Данчук, М.Р. Клюцук, Т.І. Приступа, Л.Б. Савчук // Науковий вісник НУБіП України. – 2017. – № 265. – С. 93–99.

A simple method for the isolation and purification of total lipids from animal tissues / J. Folch et al. //J biol. Chem. – 1957. – Т. 226. – №. 1. – С. 497‒509.

Жири тваринні і рослинні та олії. Приготування метилових ефірів жирних кислот: ДСТУ ISO 5509-2002 / Г. Єресько, М. Яцюта, Г. Насирова, М. Міщенко, О. Козаченко. – Увед. вперше; чинний від 2003-10-01. – К.: Держспоживстандарт України, 2003. – 22 с.

Alterations in the fatty acid composition of rat brain cells (neurons, astrocytes, and oligodendrocytes) and of subcellular fractions (myelin and synaptosomes) induced by a diet devoid of n‐3 fatty acids / J.M. Bourre, G. Pascal, G. Durand, M. Masson, O. Dumont, M. Piciotti // Journal of neurochemistry. – 1984. – № 43(2). – С. 342–348.

Смолянінов К.Б. Біологічна роль поліненасичених жирних кислот / К.Б. Смолянінов, Р.П. Параняк, В.Г. Янович // Біологія тварин. – 2002. – Т. 4, № 1–2. – С. 16–29.

Sumegi B. Kinetic advantage of the interaction between the fatty acid β-oxidation enzymes and the complexes of the respiratory chain / B. Sumegi, Z. Porpaczy, I. Alkonyi // Biochimica et Biophysica Acta (BBA)-Lipids and Lipid Metabolism. – 1991. – Т. 1081. – № 2. – С. 121‒128.

Los D.A. Murata Norio Structure and expression of fatty acid desaturases / D.A. Los // Biochimica et Biophysica Acta (BBA) Lipids and Lipid Metabolism. – 1998. – № 1394(1). – Р. 3–15. doi:10.1016/S0005-2760(98)00091-5. PMID 9767077.

Banskalieva V. Fatty acid composition of goat muscles and fat depots: a review / V. Banskalieva, T. Sahlu, A.L. Goetsch // Small Ruminant Research. – 2000. – V. 37. – P. 255–268.

Breslavec’, V.O., Shomina, N.V., Knjazjev, J.R. (2005). Influence of solutions of sodium hypochlorite and acetic acid on embryonic development and withdrawal of eggs of chickens. Poultry breeding. Kharkiv, 56, 25‒35.

Scott, T.A., Swetnam, C. (1993). Screening sanitizing agents and methods of application for hatching eggs I. Environmental and user friendliness. Journal of Applied Poultry Research. 2, № 1, 1‒6.

Khyzhnyak, S.V., Midyk, S.V., Sysoliatin, S.V. (2016). Fatty acids composition of inner mitochondrial membrane of rat cardiomyocytes and hepatocytes during hypoxia – hypercapnia. The Ukrainian Biochemical Journal. № 88(3), 92–98.

Midyk, S.V., Ushkalov, V.O., Danchuk, V.V., Sysoliatin, S.V., Nikitova, А.P. (2018). The fatty acid composition of sausages at retail market in Kyiv. Veterinary biotechnology. 32, № 2, 373–382.

[Danchuk, V.V., Kliutsuk, M.R., Prystupa, T.I., Savchuk, L.B.]. (2017). Dynamics physical activity pigs for impact and aquananohelativ micellar forms tocopherol. Scientific Herald of NULES of Ukraine. № 265, 93–99.

Folch, J. et al. (1957). A simple method for the isolation and purification of total lipids from animal tissues. J biol. Chem. 226, № 1, 497‒509.

Jeres’ko, G., Jacjuta, M., Nasyrova, G., Mishhenko, M., Kozachenko, O. (2003). DSTU ISO 5509-2002 Animal and Vegetable Fats and Oils. Preparation of Methyl Esters of Fatty Acids. Existing from 2003-10-01. Кyiv: Derzhspozhyvstandart Ukrai’ny, 22.

[Bourre, J.M., Pascal, G., Durand, G., Masson, M., Dumont, O., Piciotti, M.]. (1984). Alterations in the fatty acid composition of rat brain cells (neurons, astrocytes, and oligodendrocytes) and of subcellular fractions (myelin and synaptosomes) induced by a diet devoid of n‐3 fatty acids. Journal of neurochemistry. № 43(2), 342–348.

Smoljaninov, K.B., Paranjak, R.P., Janovych, V.G. (2002). Biological role of polyunsaturated fatty acids. Biology of animals. 4, № 1–2, 16–29.

Sumegi, B., Porpaczy, Z., Alkonyi, I. (1991). Kinetic advantage of the interaction between the fatty acid β-oxidation enzymes and the complexes of the respiratory chain. Biochimica et Biophysica Acta (BBA)-Lipids and Lipid Metabolism. 1081, № 2, 121‒128.

Los, D.A. (1998). Murata Norio Structure and expression of fatty acid desaturases. Biochimica et Biophysica Acta (BBA) Lipids and Lipid Metabolism. № 1394(1), 3–15. doi:10.1016/S0005-2760(98)00091-5. PMID 9767077.

Banskalieva, V., Sahlu, T., Goetsch, A.L. (2000). Fatty acid composition of goat muscles and fat depots: a review. Small Ruminant Research. 37, 255–268.

Published
2018-09-26
Section
Veterinary sciences