Methodical aspects of studying the bactericid properties of disinfectants
Abstract
In the article the results of improvement of methodical approaches of study of bactericidal properties of new of disinfectant preparations and realization of disinfection are presented at tuberculosis of agricultural animals. Unique methodology that allows for the short interval of time will get reliable data whether in relation to the presence of absence of bactericidal properties at disinfectant preparations taking into account biological researches is presented, and also allows to define quality of the conducted disinfection of stock-raising apartments at a tubercular infection. The proposed comprehensive methodology for the determination of tuberculocidal properties of new disinfectants and the quality control of disinfection carried out in farm animals with tuberculosis has several advantages over existing ones. The culture (suspension) method of preliminary determination of bactericidal properties of disinfectants with the use of fast-growing atypical mycobacteria of group IV according to the classification of Rhion allows reducing the time of study of disinfectants twice. The use of M. bovis as a test culture of M. tuberculosis can lead to a biological study in laboratory animals, which makes it possible to confirm the results of a culture study. As an additional protective environment of mycobacteria from the action of chemical agents, it is suggested to use a thorn, which brings the experiment closer to the real conditions of use of disinfectants. The method for assessing the effectiveness of disinfection involves the definition of both sanitary and indicative microorganisms, as well as the additional use of test cultures of fast-growing atypical mycobacteria, which makes it possible in a short time to obtain reliable data. Disinfectants DZPT-2 (2 % ‒ 24 hours), Ecocide C (5 % ‒ 24 hours) and Novodez-forte (5 % ‒ 5 hours) can be used for preventive and sanitary measures for tuberculosis of farm animals.References
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