Molecular genetic markers in сattle suffering from fusobacteriosis
Abstract
The article presents research findings of alleles of the BoLA-DRB3.2 gene, which are associated with cows suffering from fusobacteriosis (necrobacteriosis) and can serve as DNA markers of the disease. The research was carried out from 2012 to 2017 on several farms of Dunaievetskyi and Belogorsk districts in Khmelnytskyi region. Fusobacteriosis was diagnosed on the basis of epizootic, clinical, and pathoanatomical data and laboratory analysis results. Blood samples were taken from 173 healthy сows and 120 sick animals affected by fusobacteriosis. The allelic spectrum of the BoLA-DRB3.2 gene was studied with the use of PCR-RFLP. Alleles with a close relationship to susceptibility or resistance to fusobacteriosis, can be used as DNA markers; they were established by the rate of detection and risk of incidence (RR) with the Pearson criteria (χ2). The results of the research showed that the incidence of fusobacteriosis on the breeding farms of Khmelnytskyi Region ranged from 5,8 % to 15,1 % of the total number of dairy herds. In the etiology of fusobacteriosis, the leading place belongs to Fusobacterium necrophorum, isolated in 90,6 % of the samples of the investigated material. F. necrophorum has always been isolated in associations with other bacteria: staphylococcus aureus, clostridia, streptococci, escherichia, and other opportunistic pathogens. The pathogen has not always grown on nutrient media, but the biological test on rabbits has always been positive. The pure culture of Fusobacterium necrophorum was obtained precisely by biological sampling. 37 alleles with an average frequency of 2,7 % of the 54 described by PCR-RFLP method for the BoLA-DRB3.2 have been identified in cows of Ukrainian black-and-white milk breed. 7 alleles with more than 5 % frequency have been revealed in the general population: *24, *22, *08, *16, *28, *03, and *23. Rarely observed alleles include *05, *19, *20, *29, *31 and * 39. Four alleles (*16, *18, *23 and *51) that have a close proximity and three alleles (*01, *03 and *22) that are associated with the resistance to the disease have been established.References
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