Biochemical and immunological blood parameters of cows of infected by Fasciolosis and Dicrocoeliosis
Abstract
It was found that trematodaes are ones of the most dangerous and widespread helminths of ruminants according to the literature. There are the results of studies some biochemical and immunological blood parameters in cattle with parasites Fasciola hepatica and Dicrocoelium lanceatum in the article. Most biochemical parameters indicate that the main changes associated with destructive processes in the liver of cows infected by F. hepatica and D. lanceatum. The development of helminthes has toxic effect on hepatocytes, that is contributing to increase the permeability of biological membranes in cells. These changes increase the enzyme activity in serum, so we can observe a significant increase of ALT activity (P <0,05) in particular the research. It was found that Fasciola infection leads to a decrease of total protein by an average in 5,5 % and increase of bilirubin in 20,6 %. It was recorded that cholesterol was increased to 6,02 ± 0,46 (at Fasciolosis) and 5,7 ± 0,41 mmol/l (at Dicrocoeliosis) ‒ P <0,01, that is confirming the strengthening of catabolism in the body and directly indicating the destruction of hepatocytes. Helminthes have negative effect on the performance of the immune system of sick animals, showing a reduction of Ig M in 10,0 % at Fasciolosis and a 7,7 % at Dicroceliosis for chronic disease. However, the 2,5 % reduction of Ig G in group F and 1,95 % reduction in group D, compared with the control were noted. The main level of immune cells in infected cows had significant differences from that of healthy animals. So, B lymphocytes (SD22) were 11,6 ± 0,75 % (at Fasciolosis) and 11,7 ± 0,7 % (at Dicrocoeliosis) in the experimental groups. However, the figure was 14,4 ± 1,03 % in healthy cows. It is believed that the test of nitroblue tetrazolium (NBT-test) provides important information not only about the function of neutrophils, but their reaction to the toxic products of the pathological process of the body in general. It was decreased in F & D groupes (P <0,01 and P <0,05, respectively). The indicator of phagocytic index was decreased in 7,5 % (P <0,05) at Fasciola infected cows also.References
Білопольська Т.П. Епізоотологія дикроцеліозу великої рогатої худоби у Миколаївській області / Т.П. Білопольська // Науковий вісник НУБіП України. – 2010. – № 151(2). – С. 19–22.
Білопольська Т.П. Зміни у крові корів за дикроцеліозу / Т.П. Білопольська // Науковий вісник Львівського національного університету ветеринарної медицини та біотехнологій ім. Ґжицького. – 2011. – Т. 13, № 4(1). – С. 25–29.
Горячковский А.М. Справочное пособие по клинической биохимии / Горячковский А.М. – Одесса, 1994. – 416 с.
Даугалиева Э.Х. Механизм развития клеточного и гуморального иммуного ответа при гельминтозах / Э.Х. Даугалиева // Матер. докл. науч. конф. “Гельминтозоонозы – меры борьбы и профилактики”. – М., 1994. – С. 63–65.
Даугалиева Э.Х. Особенности иммунитета при гельминтозах / Э.Х. Даугалиева, К.Г. Курочкина, А.В. Арипкин // Ветеринария. – 1996. – №7. – С. 37–38.
Дахно І.С. Епізоотологія, патогенез, етіотропна та імунокоригуюча терапія при фасціольозі і дикроцеліозі жуйних тварин: автореф. дис. на здобуття наук. ступеня доктора вет. наук: спец. 03.00.18 / І.С. Дахно. – Харків, 2001. – 36 с.
Довгій Ю.Ю. Фасціольоз великої рогатої худоби в умовах тривалого іонізуючого випромінювання (епізоотологія, патогенез та лікування): автореф. дис. на здобуття наук. ступеня доктора вет. наук: спец. 16.00.11 / Ю.Ю. Довгій. – К., 2005. – 34 с.
Клінічна діагностика внутрішніх хвороб тварин / [ В.І. Левченко, В.В. Влізло, І.П. Кондрахін та ін.]. – Біла Церква, 2004. – 608 с.
Коваль І.В. Критерії оцінки якості продуктів забою тварин за фасціольозу, дикроцеліозу та ехінококозу: автореф. дис. на здобуття наук. ступеня канд. вет. наук: спец. 16.00.09 / І.В. Коваль. – Суми, 2017. – 23 с.
Куликова О.Л. Моно- и микстпаразитозы животных в среднем и нижнем Поволжье и их биологическая опасность (эпизоотологический мониторинг и меры борьбы): автореф. дис. на соискание уч. степени доктора вет. наук: спец. 03.00.19, 06.02.02 / О.Л. Куликова. – Нижний Новгород, 2010. – 51 с.
Пономар С.І. Моніторинг епізоотологічної ситуації гельмінтозів шлунково-кишкового тракту корів на території України (за даними ветеринарної статистики) / С.І. Пономар, О.В. Кручиненко // Вісник ПДАА. –2014. – № 2. – С. 116–118.
Ходжаев М.Д. Распространение фасциолеза крупного рогатого скота в Гиссарской долине Таджикистана / М.Д. Ходжаев, Ш.Ш. Разиков // Российский паразитологический журнал. – 2008. – № 4. – С. 47–49.
Шелякин И.Д. Биохимические изменения в организме крупного рогатого скота при фасциолёзе / И.Д. Шелякин, С.Н. Семёнов, О.М. Мармурова // Российский паразитологический журнал. – 2015. – № 1. – С. 53–56.
Шелякин И.Д. Изменение показателей крови коров при экспериментальном лечении фасциолеза / И.Д. Шелякин, Л.В. Ческидова // Вестник Воронежского государственного аграрного университета. – 2016. – № 1. – С. 45–50. doi: 10.17238/issn2071-2243.2016.1.45
Ahmadi-hamedani M. Evaluation of selected biochemical parameters and hepatic enzymes activity in serum of cattle naturally infected with Dicrocoelium dendriticum in Semnan Province, Iran / M. Ahmadi-hamedani // Comparative Clinical Pathology. – 2016. – № 3. – P. 555–558.
Fasciola hepatica is associated with the failure to detect bovine tuberculosis in dairy cattle / [J. Claridge, P. Diggle, C.M. McCann et al.] // Nature Communications. – 2012. – 3. – P. 853.
Howell A., Baylis M., Smith R. et al. Epidemiology and impact of Fasciola hepatica exposure in high‐yielding dairy herds / A. Howell, M. Baylis, R. Smith et al. // Preventive Veterinary Medicine – 2015. – 121. – P. 41–48.
A Retrospective Survey of Fasciolosis and Dicrocoeliosis in Slaughtered Animals in Meisam Abattoir, Tehran, Iran (2005-2008) / [A. Khanjari, R. Partovi, S. Abbaszadeh et al.] // Veterinary Research Forum. – 2010. – Vol. 1, № 3. – P. 174‒178.
Manga-González M.Y. Dicrocoeliidae family: major species causing veterinary diseases / M.Y. Manga-González, M.C. Ferreras // Advances in Experimental Medicine and Biology. – 2014. – № 766. ‒ P. 393–428. doi: 10.1007/978-1-4939-0915-5_12.
Hematological and biochemical changes in organically farmed sheep naturally infected with Fasciola hepatica / [K. Matanović, K. Severin, F. Martinković et al.] // Parasitol Res. – 2007. – № 101(6). – Р. 1657‒1661. doi: 10.1007/s00436-007-0709-2.
Samadieh H. Relationships between Oxidative Stress, Liver, and Erythrocyte Injury, Trace Elements and Parasite Burden in Sheep Naturally Infected with Dicrocoelium dendriticum. / [H. Samadieh, G-R. Mohammadi, M. Maleki et al.] // Iran J. Parasitology. – 2017. – № 12(1). – P. 46–55.
Bilopolska, T.P. (2010). Epizootology of dicrocoeliosis of cattle in the Mykolaiv region. Scientific herald of nules of Ukraine. Series: veterinary medicine, quality and safety of products of stock-raising. № 151(2), 19–22.
Bilopolska, T.P. (2011). Changes in the blood of cows for dicrocoeliosis. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnology. 13, № 4(1), 25–29.
Goryachkovskiy, A.M. (1994). Reference book on clinical biochemistry. Odessa, 416.
Daugalieva, E.H. (1994). The mechanism of development of cellular and humoral immune response in helminthiases. Materials doc. sci. сonf. “Helminthazonosis measures of control and prevention”. Мoskow, 63–65.
Daugalieva, E.H. Kurochkina, K.G., Aripkin, A.V. (1996). Features of immunity in helminthiases. Veterinary medicine. № 7, 37–38.
Dakhno, I.S. (2001). Epizootology, pathogenesis, etiotrophia and immunocorrelation therapy in fasciolosis and dicroceliosis of ruminants. The avtoref. dissertation to compete the academic degree of Doctor’s Veterinary Science. Kharkiv, 36.
Dovhii, Y.Y. (2005). Fasciolosis of cattle in conditions of long-term ionizing radiation (epizootology, pathogenesis and treatment). The avtoref. dissertation to compete the academic degree of Doctor’s Veterinary Science. Кyiv, 34.
[Levchenko, V.I., Vlizlo, V.V., Kondrakhin, I.P. et al.]. (2004). Clinical diagnosis of internal animal diseases. Bila Tserkva, 608.
Koval, I.V. (2017). Сriteria for the assessment of products of slauqhter animals fascioles, dycrocelias and echinococcus. The avtoref. dissertation to compete the academic degree of ph. D. Sumy, 23.
Kulikova, O.L. (2010). Mono- and mixed parasitosis of animals in the middle and lower Volga region and their biological danger (epizootic monitoring and control measures). The avtoref. dissertation to compete the academic degree of Doctor’s Veterinary Science. Nizhny Novgorod, 51.
Ponomar, S.I., Kruchynenko, O.V. (2014). Monitoring of the epizootological situation of helminthiasis of the gastrointestinal tract of cows in Ukraine (according to veterinary statistics). Bulletin of Poltava state agrarian academy. № 2, 116–118.
Hodzhaev, M.D., Razikov, Sh.Sh. (2008). Distribution of fasciolosis of cattle in the Gissar valley of Tajikistan. Russian Journal of Parasitology. № 4, 47–49.
Shelyakin, I.D., Semyonov, S.N., Marmurova, O.M. (2015). Biochemical changes in cattle in fascioliasis. Russian Journal of Parasitology. № 1, 53–56.
Shelyakin, I.D., Cheskidova, L.V. (2016). Change in cows blood levels during the experimental treatment of fascioliasis. Vestnik Of Voronezh State Agrarian University. № 1, 45–50. doi: 10.17238/issn2071-2243.2016.1.45
Ahmadi-hamedani, M. (2016). Evaluation of selected biochemical parameters and hepatic enzymes activity in serum of cattle naturally infected with Dicrocoelium dendriticum in Semnan Province, Iran. Comparative Clinical Pathology, 3, 555–558.
[Claridge, J., Diggle, P., McCann, C.M., Mulcahy, G., Flynn, R., McNair, J., Williams, D.J.L.] (2012). Fasciola hepatica is associated with the failure to detect bovine tuberculosis in dairy cattle. Nature Communications. 3, 853.
[Howell, A., Baylis, M., Smith, R., Pinchbeck, G., Williams, D.] (2015). Epidemiology and impact of Fasciola hepatica exposure in high‐yielding dairy herds. Preventive Veterinary Medicine, 121, 41–48.
[Khanjari, A., Partovi, R., Abbaszadeh, S. et al.]. (2010). A Retrospective Survey of Fasciolosis and Dicrocoeliosis in Slaughtered Animals in Meisam Abattoir, Tehran, Iran (2005‒2008). Veterinary Research Forum. № 1(3), 174‒178.
Manga-González, M.Y. (2014). Dicrocoeliidae family: major species causing veterinary diseases. Advances in Experimental Medicine and Biology. 766, 393–428. doi: 10.1007/978-1-4939-0915-5_12.
[Matanović, K. Severin, K., Martinković, F. et al.]. (2007). Hematological and biochemical changes in organically farmed sheep naturally infected with Fasciola hepatica. Journal of Parasitology Research. № 101(6), 1657‒1661. doi: 10.1007/s00436-007-0709-2.
Samadieh, H., Mohammadi, G-R., Maleki, M. (2017). Relationships between Oxidative Stress, Liver, and Erythrocyte Injury, Trace Elements and Parasite Burden in Sheep Naturally Infected with Dicrocoelium dendriticum. Iran Journal Parasitology. № 12(1), 46–55.
