Peroxidase activity and isoenzyme composition in Robinia pseudoacacia L. leaves under the influence of Parectopa robiniella Clemens, 1863

Keywords: R. pseudoacacia L. leaves; Parectopa robiniella; bensidine- and guajacol-peroxidase activity; isoenzyme expressivity.

Abstract

Іn recent times R. pseudoacacia has been suffering from populations of new pests, which cause serious economic damage to this strategic species. Therefore, studying the features of plant defense responses against harmful insects is of great importance for biodiversity conservation for preserving this species in natural and artificial plantings. The aim of the study was to analyze the peculiarities of the activity and isoenzyme composition of peroxidase in R. pseudoacacia leaves during attacks by the invading insect P. robiniella. In the green plantations of Dnipro city, as the object of research, six groups of Robinia pseudoacacia L. model trees of different ages (5 and 35–40 years of age) with the existing leaf damages by P. robiniella leaf blotch miner were identified. Differences in benzidine-peroxidase activity were found in the leaves of 5- and 40-year-old R. pseudoacacia trees. In 35‒40-year-old trees with the presence of leaf blotch mi­ners, the leaves showed a tendency to increase BPOD activity by 24.6% vs control. While in young plants, a significant increase in peroxidase activity was found by 3.8 times due to mechanical damage of the leaves by the miner compared to the control. The revealed significant increase (by 63.2%) in the activity of guaiacol-peroxidase of black locust indicates its active participation in enhancing the barrier functions of leaf cells against P. robiniella, and it indicates the promising use of the enzyme as a biomarker for pest influence. The composition of benzidine-peroxidase isoenzymes was analyzed by electrofocusing. The main pattern of changes in the expressiveness of benzidine-peroxidase due to damage of leaves by a pest was a quantitative redistribution of activity between different molecular forms of peroxidase. Several enzyme isoforms from the highly acidic pH zone showed a significant activity increase in R. pseudoacacia leaves damaged by P. robiniella; this especially applies to the peroxidase system of young black locust plants. The increase in bensidine- and guajacol-peroxidase activity under the influence of leaf miner may be explained by a change in the gene expression of individual isoforms of peroxidases, which is confirmed by the results of studying the relative share of peroxidase molecular forms with isoelectrofocusing. Calculation of the internal diversity index (KD) of IEF benzidine-peroxidase spectra showed that the studied peroxidase spectra of young and medieval trees of control and experimental samples of R. pseudoacacia leaves differ significantly in the level of complexity. Under the influence of the invader the level of complexity increased. The study of the reaction of black locust and the mechanisms of its protection against mechanical damage by phytophages with the involvement of a larger range of enzymes in further research can serve as a theoretical basis for species selection and management to restore it under biotic stress.

References

Bongarten B.C., Huber D.A. & Apsley D.K. (1992). Environmental and genetic influences on short-rotation biomass production of black locust (Robinia pseudoacacia L.) in the Georgia Piedmont. For. Ecol. Manage. 55, 315–331.

Davis D.R., De Prins J. (2011). Systematics and biology of the new genus Macrosaccus with descriptions of two new species (Lepidoptera, Gracillariidae). ZooKeys, 98, 29–82.

Dini-Papanastasi О. and Aravanopoulos F.A. (2008). Artificial hybridization between Robinia pseudoacacia L. and R. pseudoacacia var. monophylla Carr. Forestry, 81(1), 91–101. doi: 10.1093/forestry/cpm047

Enescu, C.M.; Dӑnescu, A. (2013). Black locust (Robinia pseudoacacia L.) – an invasive neophyte in the conventional land reclamation flora in Romania. Bulletin of the Transilvania University of Braşov Series II, Forestry, Wood Industry, Agricultural Food Engineering, 6(55), 2, 24–30.

Gill, R. S., Gupta, K., Taggar, G. K., & Taggar, M. S. (2010). Role of oxidative enzymes in plant defenses against herbivory. Acta Phytopathologica et Entomologica Hungarica, 45(2), 277–290.

Gregory, R.P.F. (1966). А rаpid аssаy for peroxidаse аctivity. Biochem. J., 101 (3), 582–583.

Guo, X., Ren, X., Elle,r F. (2018) Higher phenotypic plasticity does not confer higher salt resistance to Robinia pseudoacacia than Amorpha fruticosa. Acta Physiol Plant, 4, 40–79. doi: 10.1007/s11738-018-2654-3

He, J., Chen, F., Chen, S., Lv, G., Deng, Y., Fang, Z., Guan, Z., He, C. (2011). Chrysanthemum leaf epidermal surface morphology and antioxidant and defense enzyme activity in response to aphid infestation. Journal of Plant Physiology, 168, 687–693.

Holoborodko, K.K., Marenkov, O.M., Gorban, V.A., Voronkova, Y.S. (2016). The problem of assessing the viability of invasive species in the conditions of the steppe zone of Ukraine. Vìsn. Dnìpropetr. Unìv. Ser. Bìol. Ekol, 24(2), 466–472.

Kirichenko, N., Augustin, S. and Kenis, M. (2018). Invasive leafminers on woody plants: a global review of pathways, impact, and management. Journal of Pest Science, 92, 93–106. doi 10.1007/s10340-018-1009-6

Oszmian,´ski J, Kalisz, S, Wojdyło, A (2014) The content of phenolic compounds in leaf tissue of white (Aesculus hippocastanum L.) and red horse chestnut (Aesculus carnea H.) colonized by the horse chestnut leaf miner (Cameraria ohridella Deschka & Dimic´). Molecules, 19, 14625–14636. doi:10.3390/molecules190914625

Paterska, M., Bandurska, H., Wysłouch, J., Molińska-Glura, M., Moliński, K. (2017). Chemical composition of horse-chestnut (Aesculus) leaves and their susceptibility to chestnut leaf miner Cameraria ohridella Deschka & Dimić. Acta Physiol. Plant, 39(105), 1–16. doi: 10.1007/S11738-017-2404-Y

Pentelyuk, O.S., Lihanov, A.F., Hryhoryuk, I.P. (2016). Dynamics of polyphenols content in the leaves of bitter chestnut (Aesculus hippocastanum L.) under conditions of mechanical damage. Bioresursy i pryrodokorystuvannya, 1–2, 5–12 (in Ukranian).

Sjöman, H, Hirons, A.D., Bassuk, N.L..(2015). Urban forest resilience through tree selection–Variation in drought tolerance in Acer. Urban Forestry & Urban Greening, 14(4), 858–865.

Sánchez-Sánchez, H. & Morquecho-Contreras, A. (2013). Chemical Plant Defense against Herbivores. Herbivores, Vonnie D. C. Shields, IntechOpen. Chapter 1, 3–29. doi: 10.5772/6734619

Singh, H., Dixit, S., Verma, P. C., Singh, P. K. (2013). Differential peroxidase activities in three different crops upon insect feeding. Plant Signal and Behaviour, 8(10), 1–7.

Shupranova, L.V., Holoborodko, K.K., Seliutina, O.V., Pakhomov, O.Y. (2019). The influence of Cameraria ohridella (Lepidoptera, Gracillariidae) on the activity of the enzymatic antioxidant system of protection of the assimilating organs of Aesculus hippocastanum in an urbogenic environment. Biosyst. Divers., 27(3), 238–243. doi: 10.15421/011933

Takashima, Y., Suzuki, M., Ishiguri, F., Iizuka, K., Yoshizawa, N., Yokota, S. (2013). Cationic peroxidase related to basal resistance of Betula platyphylla var. japonica plantlet No. 8 against canker-rot fungus Inonotus obliquus strain IO-U1. Plant Biotechnology, 30(2), 199–205. doi: 10.5511/plantbiotechnology.13.0312b

Toptikov, V.A., Dyachenko, L.F., Totsky, V.N. (2010). Assessment of the spectra of multiple forms of enzymes using an index of internal diversity. Cytology and genetics, 44(1): 46–53.

Vítková, M., Müllerová, J., Sádlo, J. et al. (2017). Black locust (Robinia pseudoacacia) beloved and despised: A story of an invasive tree in Central Europe. Forest Ecology and Management, 384, 287–302. doi: 10.1016/j.foreco.2016.10.057

War, A. R., Taggar, G. K., Hussain, B., Taggar, M. S., Nair, R. M., Sharma, H. C. (2018). Plant defence against herbivory and insect adaptations. AoB PLANTS, 10(4).

Wilkaniec, A., Borowiak-Sobkowiak, B., Irzykowska, L., Breś, W., Świerk, D., Pardela, L., Durak, R., Środulska-Wielgus, J. Wielgus, K. (2021). Biotic and abiotic factors causing the collapse of Robinia pseudoacacia L. veteran trees in urban environments. PLoS One, 16(1). doi: 10.1371/journal.pone.0245398

Wojda, T, Klisz, M, Jastrzebowski, S, Mionskowski, M, Szyp-Borowska, I, Szczygiel, K. (2015). The geographical distribution of the black locust (Robinia pseudoacacia L.) in Poland and its role on non-forest land. Papers on Global Change, 22, 101–113.

Published
2021-11-15
How to Cite
Shupranova, L., Holoborodko, K., Guslistiy, A., Shulman, M., & Pakhomov, O. (2021). Peroxidase activity and isoenzyme composition in Robinia pseudoacacia L. leaves under the influence of Parectopa robiniella Clemens, 1863. Agrology, 4(4), 174-179. https://doi.org/10.32819/021020
Section
Оriginal researches