Estimation of the resistance of maize lines to Sphacelotheca reiliana in the northern Steppe of Ukraine

Keywords: Zea mays; inbreds; head smut; disease susceptibility; selection; artificial infective bachground

Abstract

Maize selection for disease resistance is an actual task owing to the expansion of phytopathogens under climate changes and maize production in monoculture. The aim of the research was to differentiate modern maize initial breeding material by susceptibility to causative agent of head smut (Sphacelotheca reiliana) in the conditions of the northern Steppe of Ukraine. 28 maize inbreds from the collection of SE The Institute of Grain Crops of NAAS were taken for estimation of resistance to head smut in contrasting 2018 and 2019 on artificial infectious background in Dnipropetrovsk region. According to the results of the cultivation, all tested samples were divided into four classes on the level of susceptibility to disease: stable, low sensitive, middle sensitive and unstable. In 2018, 11 lines showed low sensitivity, 8 lines – middle and 9 lines – high sensitivity to head smut. In 2019 – no signs of pathogenesis were detected in 12 lines while 6 inbreds were classified as low sensitive, 7 – middle sensitive and 3 – high sensitive. The largest amount of stable or low sensitive to S. reiliana lines was among those ones originated from Lancaster germplasm. Among 9 lines of Iodent germplasm in 2018‒2019 inbred DK 742 was assigned to the 2nd class of sensitivity; DK315 and DK744 in 2018 – to 4th and 3rd classes while in 2019 – to 2nd and 1st classes, respectively. Five other Iodent lines were either unstable or had intermediate susceptibility to the pathogen. As to Reid germplasm in two research years DK239MV showed the sensitivity of 1.6‒9.0% and was classified as low sensitive; DK2323 was determined as intermediate sensitive in 2018 and high sensitive in 2019. In arid conditions of 2018 up to 20% of DK367 plants had typical signs of infection while in wet 2019 this line had no signs of infection by S. reiliana at all. Flint inbred DK4538 appeared unstable in both years. Other inbreds with flint grain type DK959 and DK276 in two years varied greatly on their resistance. DK 247MV which was related to line Co125 had low susceptibility to the pathogen at the level of 4.7‒5.6%. DK129-4, also related to Co125, demonstrated susceptibility from 0 to 18.3%. Since maize resistance to head smut is a polygenic trait, it largely depends on weather conditions, as well as the virulence of the pathogen, the ability of plants to prevent pathological processes at the level of histogenesis, fungistatic effects of soil microorganisms and maize root secretions. Taking into account the influence of the external environment, the revealed perspective lines with ability to withstand the head smut in the northern Steppe of Ukraine can be used in the selection process of maize hybrids resistant to S. reiliana

References

Ali, M., Ahmad, Z., Ashraf, M., & Dong, W. (2020). Maize endophytic microbial-communities revealed by removing PCR and 16S rRNA sequencing and their synthetic applications to suppress maize banded leaf and sheath blight. Microbiological Research, 242, 126639. doi: 10.1016/j.micres.2020.126639

Álvarez-Cervantes, J., Hernández-Domínguez, E. M., Tellez-Tellez, M., Mandujano-González, V., Mercado-Flores, Y., & Díaz-Go-dinez, G. (2016) Stenocarpella maydis and Sporisorium reilianum: Two Pathogenic Fungi of Maize. Fungal Pathogenicity (S. Sultan ed.), 45–60. doi: 10.5772/62662

Bannikova, K., & Yavdoshchenko, M. (2016). Maize diseases in 2015 and the forecast of their spread in 2016. Propozytsiia, 3, 35–38 (in Ukrainian).

Baranova, V. V., Petrenkova, V. P., Chernobai, L. M., & Borovska, I. Y. (2012). Inheritance of a sign of resistance of corn to the pathogen of head smut. Plant Breeding and Seed Production, 101, 123–130 (in Ukrainian).

Brereton, R. G. (2007). Applied Chemometrics for Scientists. Wiley, Chichester, UK.

Chen, Y., Chao, Q., Tan, G., Zhao, J., Zhang, M., Ji, Q., & Xu, M. (2008). Identification and fine-mapping of a major QTL conferring resistance against head smut in maize. Theor Appl Genet., 117(8), 1241–1252. doi: 10.1007/s00122-008-0858-4

Dermenko, O. M. (2012). Smut`s diseases of maize. Propozytsiia, 8, 76–78 (in Ukrainian).

Di, H., Yu, T., Deng, Y., Dong, X., Li, R., Zhou, Y., & Wang, Z. (2017). Complementary DNA (cDNA) cloning and functional verification of resistance to head smut disease (Sphacelotheca reiliana) of an NBS–LRR gene ZmNL in maize (Zea mays). Euphytica, 213, 288. doi: 10.1007/s10681-017-2083-1

Dutra, D., Agrawal, N., Ghareeb, H., & Schirawski, J. (2020). Screening of Secreted Proteins of Sporisorium reilianum f. sp. zeae for Cell Death Suppression in Nicotiana benthamiana. Frontiers in Plant Science, 11. doi: 10.3389/fpls.2020.00095

Ge, Ch., Tang, Ch., Zhu, Y., & Wang, G. (2020). Genome-wide identification of the maize 2OGD superfamily genes and their response to Fusarium verticillioides and Fusarium grami-

nearum. Gene, 764, 145078. doi: 10.1016/j.gene.2020.145078

Ghareeb, H., Zhao, Y., & Schirawski, J. (2019). Sporisorium rei-

lianum possesses a pool of effector proteins that modulate virulence on maize. Mol Plant Pathol., 20(1), 124–136. doi: 10.1111/mpp.12744

Ivashchenko, V. G. (2015). Maize Diseases: Etiology, Monitoring and Problems of Variety Resistance. St. Peterburg (Plant Protection News, Suppl.) (in Russian).

Jiang, S., Cheng, Q., Yan, J., Fu, R., & Wang, X. (2020). Genome optimization for improvement of maize breeding. Theor Appl Genet., 133(5), 1491‒1502. doi: 10.1007/s00122-019-03493-z

Lebid, Y. M., Tsykov, V. S., & Pashchenko, Y. M. (2008). Methods of conducting field experiments with maize. Dnipropetrovsk (in Ukrainian).

Li, Y.-X., Wu, X., Jaqueth, J., Zhang, D., Cui, D., Li, C. … Li, Y. (2015). The Identification of Two Head Smut Resistance-Related QTL in Maize by the Joint Approach of Linkage Mapping and Association Analysis. PLOS ONE, 10(12), e0145549. doi: 10.1371/journal.pone.0145549

Lugtenberg, B. J., Caradus, J. R., & Johnson, L. J. (2016). Fungal endophytes for sustainable crop production. FEMS Microbiol Ecol., 92(12), 194. doi: 10.1093/femsec/fiw194

Markov, I. L. (2011) Diagnose maize diseases. Agribusiness today, 5(204), 37–42 (in Ukrainian).

Neer, E. J., Schmidt, C. J., Nambudripad, R., & Smith, T. F. (1994). The ancient regulatory-protein family of WD-repeat proteins. Nature, 371(6495), 297–300. doi: 10.1038/371297a0

Nyaga, C., Gowda, M., Beyene, Y., Murithi, W.T., Burgueno, J., Toledo, F. … Prasanna, B.M. (2020). Hybrid Breeding for MLN Resistance: Heterosis, Combining Ability, and Hybrid Prediction. Plants (Basel), 9(4), 468. doi: 10.3390/plants9040468

Poloni, A., & Schirawski, J. (2016). Host specificity in Sporisorium reilianum is determined by distinct mechanisms in maize and sorghum. Mol Plant Pathol., 17(5), 741–754. doi: 10.1111/mpp.12326

Schirawski, J., Mannhaupt, G., Münch, K., Brefort, T., Schipper, K., Doehlemann, G. … Kahmann, R. (2010). Pathogenicity determinants in smut fungi revealed by genome comparison. Science, 330(6010), 1546–1548. doi: 10.1126/science.1195330

Schweizer, G., Münch, K., Mannhaupt, G., Schirawski, J., Kahmann, R., & Dutheil, J.Y. (2018). Positively Selected Effector Genes and Their Contribution to Virulence in the Smut Fungus Sporisorium reilianum. Genome Biol Evol., 10(2), 629‒645. doi: 10.1093/gbe/evy023

Tatarynova, V. I., Rozhkova, T. O., Burdulaniuk, A. O., & Vasylyna, M. I. (2015) Resistance of maize hybrids to smut diseases. Visnyk of Sumy National Agrarian University, 9, 108–111 (in Ukrainian).

Wagner, M. R., Busby, P. E., & Balint-Kurti, P. (2020). Analysis of leaf microbiome composition of near-isogenic maize lines differing in broad-spectrum disease resistance. New Phytol., 225(5), 2152–2165. doi: 10.1111/nph.16284

Wang, B., Lin, Z., Li, X., Zhao, Y., Zhao, B., Wu, G. … Wang, H. (2020). Genome-wide selection and genetic improvement during modern maize breeding. Nat Genet., 52(6), 565–571. doi: 10.1038/s41588-020-0616-3

Wang, M., Yan, J., Zhao, J., Song, W., Zhang, X., Xiao, Y., & Zheng Y. (2012). Genome-wide association study (GWAS) of resis-

tance to head smut in maize. Plant Science. 1–7. doi: 10.1016/j.plantsci.2012.08.004

Weng, J., Liu, X., Wang, Z., Wang, J., Zhang, L., Hao, Z., Xie, C., Li, M., Zhang, D., Bai, L., Liu, C., Zhang, S., & Li, X. (2012). Molecular mapping of the major resistance quantitative trait locus qHS2.09 with simple sequence repeat and single nucleotide polymorphism markers in maize. Phytopathology, 102(7), 692–699. doi: 10.1094/PHYTO-12-11-0330

West, A. G., Shore, P., & Sharrocks, A. D. (1997). DNA binding by MADS-box transcription factors: a molecular mechanism for differential DNA bending. Molecular Cell Biology, 17(5), 2876–2887.

Wu, C. H., Abd-El-Haliem, A., Bozkurt, T. O., Belhaj, K., Te-rauchi, R., Vossen, J. H., & Kamoun, S. (2017). NLR network mediates immunity to diverse plant pathogens. Proc Natl Acad Sci USA, 114(30), 8113–8118. doi: 10.1073/pnas.1702041114

Yu, K., Wang, H., Liu, X., Xu, C., Li, Z., Xu, X., Liu, J., Wang, Z., & Xu, Y. (2020). Large-Scale Analysis of Combining Abi-

lity and Heterosis for Development of Hybrid Maize Breeding Strategies Using Diverse Germplasm Resources. Front Plant Sci., 11, 660. doi: 10.3389/fpls.2020.00660

Yunikov, V. G. (1969). To the study of head smut of maize in the Voronezh region. Agricultural science – production, 167–176 (in Russian).

Yurku, A. I. (1990). Maize head smut: monograph]. Shtiintsa: Kishinev (in Russian).

Zhang, M., Cui, Y., Liu, Y.H., Xu, W., Sze, S. H., Murray, S. C., Xu, S., & Zhang, H. B. (2020). Accurate prediction of maize grain yield using its contributing genes for gene-based breeding. Genomics, 112(1), 225–236. doi: 10.1016/j.ygeno.2019.02.001

Zhang, N., Zhang, B., Zuo, W., Xing, Y., Konlasuk, S., Tan, G., Zhang, Q., Ye, J., & Xu, M. (2017). Cytological and Molecular Characterization of ZmWAK-Mediated Head-Smut Resistance in Maize. Mol Plant Microbe Interact., 30(6), 455–465. doi: 10.1094/MPMI-11-16-0238-R

Zhao, X., Tan, G., Xing, Y., Wei, L., Chao, Q., Zuo, W., Lübberstedt, T., & Xu, M. (2012). Marker-assisted introgression of qHSR1 to improve maize resistance to head smut. Mol Breeding, 30(2), 1077–1088. doi: 10.1007/s11032-011-9694-3

Zuo, W., Chao, Q., Zhang, N., Ye, J., Tan, G., Li, B. … Xu, M. (2015). A maize wall-associated kinase confers quantitative resistance to head smut. Nat Genet., 47(2), 151–157. doi: 10.1038/ng.3170

Zuo, W., Ökmen, B., Depotter, J. R. L., Ebert, M. K., Redkar, A., Misas Villamil, J., & Doehlemann, G. (2019). Molecular Interactions Between Smut Fungi and Their Host Plants. Annu Rev Phytopathol., 57, 411–430. doi: 10.1146/annurev-phyto-082718-100139

Published
2020-09-14
How to Cite
Cherchel, V., Stasiv, O., Satarova, T., Zatyshniak, O., & Lazarev, E. (2020). Estimation of the resistance of maize lines to Sphacelotheca reiliana in the northern Steppe of Ukraine. Agrology, 3(4), 199-204. https://doi.org/10.32819/020023
Section
Оriginal researches